Husain K
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.
Biochem Int. 1991 Aug;24(6):1051-6.
Neurotoxicesterase (NTE) activity was assayed in platelets of human and mice as well as in the brain of mice in vitro and in vivo. Mipafox, a well known organophosphate, to induce delayed neurotoxicity, at doses of 5, 10 and 15 mg/kg, subcutaneously, was used to examine the relationship between inhibition of brain and platelet NTE activity in mice. It was observed that the platelet NTE activity of mice was less than in humans. The optimum pH for both brain and platelet NTE of mice, and human platelets, was 8. The results indicate that mipafox produces a dose dependent inhibition of brain and platelet NTE activity in vivo and concentration dependent inhibition in vitro. It can be concluded that assay of platelet NTE can be a useful peripheral biochemical marker for organophosphate-induced delayed neurotoxicity.
在体外和体内对人和小鼠的血小板以及小鼠的大脑进行了神经毒素酯酶（NTE）活性测定。使用已知可诱导迟发性神经毒性的有机磷酸酯丙氟磷，以5、10和15mg/kg的剂量皮下注射，来研究小鼠大脑和血小板NTE活性抑制之间的关系。观察到小鼠的血小板NTE活性低于人类。小鼠大脑和血小板以及人类血小板的NTE的最适pH均为8。结果表明，丙氟磷在体内对大脑和血小板NTE活性产生剂量依赖性抑制，在体外产生浓度依赖性抑制。可以得出结论，血小板NTE测定可作为有机磷酸酯诱导的迟发性神经毒性的有用外周生化标志物。
