ras p21在结直肠癌中的表达与DNA倍体、S期分数及预后的关系

ras p21 expression in relation to DNA ploidy, S-phase fraction and prognosis in colorectal adenocarcinoma.

作者信息

Sun X F, Wingren S, Carstensen J M, Stål O, Hatschek T, Boeryd B, Nordenskjöld B, Zhang H

机构信息

Department of Oncology, University Hospital, Linköping, Sweden.

出版信息

Eur J Cancer. 1991;27(12):1646-9. doi: 10.1016/0277-5379(91)90437-i.

DOI:10.1016/0277-5379(91)90437-i

PMID:1782077

Abstract

ras p21 expression, as indicated by the monoclonal antibody ras 11, was estimated using immunohistochemistry on 69 primary colorectal adenocarcinomas. Also, DNA ploidy and S-phase fraction (SPF) were analysed with flow cytometry. Positive staining for ras 11 tended to be more common in DNA non-diploid tumours (P = 0.11), but was significantly correlated with high SPF (P = 0.038). Positive ras 11 staining, Dukes' stage, DNA ploidy and SPF were related to the recurrence-free interval of patients with Dukes' A-C tumours (P = 0.0014, P = 0.023, P = 0.035 and P = 0.040, respectively). ras 11 staining was a prognostic factor independent of both Dukes' stage and DNA ploidy (P = 0.011). The results indicate that pan ras p21 expression is associated with proliferative activity and has an independent prognostic value in colorectal adenocarcinoma.

摘要

利用单克隆抗体ras 11检测69例原发性结肠直肠癌组织中的ras p21表达，采用免疫组织化学方法进行评估。此外，通过流式细胞术分析DNA倍体和S期细胞分数（SPF）。ras 11阳性染色在DNA非二倍体肿瘤中更常见（P = 0.11），但与高SPF显著相关（P = 0.038）。ras 11阳性染色、Dukes分期、DNA倍体和SPF与Dukes A - C期肿瘤患者的无复发生存期相关（分别为P = 0.0014、P = 0.023、P = 0.035和P = 0.040）。ras 11染色是独立于Dukes分期和DNA倍体的预后因素（P = 0.011）。结果表明，泛ras p21表达与增殖活性相关，在结肠直肠癌中具有独立的预后价值。