Sun X F, Carstensen J M, Stål O, Zhang H, Nilsson E, Sjödahl R, Nordenskjöld B
Department of Oncology, University Hospital of Linköping, Sweden.
Virchows Arch A Pathol Anat Histopathol. 1993;423(6):443-8. doi: 10.1007/BF01606533.
p53 expression, DNA ploidy and S-phase fraction were analysed retrospectively in colorectal adenocarcinomas from 293 patients in whom the long-term outcome was known. The frequency of nuclear p53 staining was increased in non-diploid tumours (42%) when compared with diploid tumours (33%). Cytoplasmic p53 positive tumours were more common in the proximal colon (32%) than in the distal sites (21%). In univariate survival analysis, nuclear p53 and cytoplasmic staining were significantly associated with poor prognosis in patients with Dukes' A-C tumours. The patients showing both nuclear and cytoplasmic p53 staining had the poorest survival and the patients with tumours negative in both the nucleus and cytoplasm showed the best prognosis. The patients with tumours positive in the nucleus alone or in the cytoplasm alone presented an intermediate survival. In multivariate survival analyses, nuclear p53 expression, cytoplasmic p53 expression and DNA ploidy were prognostic indicators independent of Dukes' stage and each other. Further analysis suggested that the prognostic importance of cytoplasmic p53 expression was greater in diploid than in non-diploid tumours. We conclude that nuclear p53 expression, cytoplasmic p53 expression and DNA ploidy provide important prognostic information in colorectal adenocarcinomas.
对293例已知长期预后的结肠腺癌患者的肿瘤组织进行回顾性分析，检测p53表达、DNA倍体及S期细胞比例。非二倍体肿瘤细胞核p53染色阳性率（42%）高于二倍体肿瘤（33%）。细胞质p53阳性肿瘤在近端结肠（32%）比远端部位（21%）更常见。单因素生存分析显示，核p53和细胞质染色与Dukes' A - C期肿瘤患者的预后不良显著相关。核和细胞质p53染色均阳性的患者生存率最差，而核和细胞质均阴性的患者预后最佳。仅细胞核或仅细胞质p53染色阳性的患者生存率居中。多因素生存分析显示，核p53表达、细胞质p53表达和DNA倍体是独立于Dukes分期且相互独立的预后指标。进一步分析表明，细胞质p53表达在二倍体肿瘤中的预后重要性高于非二倍体肿瘤。我们得出结论，核p53表达、细胞质p53表达和DNA倍体为结肠腺癌提供了重要的预后信息。
