基于多克隆抗体的时间分辨荧光免疫分析法与液相色谱-串联质谱法测定鸡肉组织中己烯雌酚残留量的比较。
Comparison of time-resolved fluoroimmunoassay for determining hexoestrol residues in chicken muscle tissues based on polyclonal antibodies with liquid chromatography and tandem mass spectrometry.
作者信息
Xiangqian Li, Yongming Hu, Tieming Huo, Xiaogang Chu, Chuanlai Xu
机构信息
Department of Chemical and Bioengineering, Huaiyin Institute of Technology, 223001, Huai'an, JiangSu Province, China.
出版信息
Ann Chim. 2007 Mar-Apr;97(3-4):177-86. doi: 10.1002/adic.200790003.
A time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of hexoestrol (HES) residues in animal tissues. The limit of detection (LOD) was determined to be 0.02 ng g(-1) and the limit of quantification (LOQ) was less than 0.12 ng g(-1). The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of market chicken muscle tissues and confirmed by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). This proposed technique could be applied to routine residue analysis.
建立了一种时间分辨荧光免疫分析法（TR-FIA）用于测定动物组织中的己烯雌酚（HES）残留。检测限（LOD）确定为0.02 ng g(-1)，定量限（LOQ）小于0.12 ng g(-1)。TR-FIA和酶联免疫吸附测定法（ELISA）所得结果具有良好的相关性。所建立的TR-FIA经用于市售鸡肉肌肉组织的测定验证，并通过高效液相色谱和串联质谱法（LC-MS-MS）得以确认。该提议的技术可应用于常规残留分析。
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