Borel N, Janett F, Teankum K, Zlinszky K, Iten C, Hilbe M
Institute of Veterinary Pathology, University of Zurich, Winterthurerstrasse, Zurich, Switzerland.
J Comp Pathol. 2007 Aug-Oct;137(2-3):169-73. doi: 10.1016/j.jcpa.2007.06.002.
A bull aged 16 months with bilateral testicular hypoplasia and azoospermia was persistently infected with bovine viral diarrhoea virus (BVDV). Viral antigen was detected in serum and semen by ELISA, but the animal was serologically negative. After slaughter, the genital tract was examined histopathologically and by immunohistochemistry, including double immunolabelling with BVDV antibody and either S-100 antibody (for Sertoli cells) or ferritin antibody (for Leydig cells). The seminiferous tubules of both testes were lined by a single layer of Sertoli cells and the germinal epithelium was completely absent except for a few remaining spermatogonia. BVDV antigen was demonstrated (1) in the media of arterial vessel walls of the testis, epididymis, urethra, prostate, and vesicular and bulbourethral glands, (2) in epithelial cells of the ductus epididymidis, the accessory glands and the urethra, and (3) in the testis, mainly in Sertoli cells and to a lesser extent in the spermatogonia that remained, but not in Leydig cells. The testicular hypoplasia was possibly linked to the BVDV infection.
一头16月龄的公牛患有双侧睾丸发育不全和无精子症，持续感染牛病毒性腹泻病毒（BVDV）。通过ELISA在血清和精液中检测到病毒抗原，但该动物血清学检测呈阴性。屠宰后，对生殖道进行了组织病理学检查和免疫组织化学检查，包括用BVDV抗体与S-100抗体（用于支持细胞）或铁蛋白抗体（用于间质细胞）进行双重免疫标记。两侧睾丸的生精小管由单层支持细胞衬里，除了少数残留的精原细胞外，生精上皮完全缺失。BVDV抗原在以下部位被证实：（1）睾丸、附睾、尿道、前列腺以及精囊腺和尿道球腺的动脉血管壁中层；（2）附睾管、附属腺和尿道的上皮细胞；（3）睾丸中，主要在支持细胞中，在残留的精原细胞中也有少量存在，但在间质细胞中未检测到。睾丸发育不全可能与BVDV感染有关。
