Bossio Joseph P, Harry Jason, Kinney Chad A
Department of Chemistry/Biochemistry, Eastern Washington University, Cheney, WA 99004-2440, USA.
Chemosphere. 2008 Jan;70(5):858-64. doi: 10.1016/j.chemosphere.2007.06.088. Epub 2007 Sep 5.
The objective of this research was the development, optimization, and demonstration of an ultrasonic assisted extraction (UAE) based method for organic anthropogenic waste indicators (AWIs) with a range of physicochemical properties from soil and sediment samples. The optimized method was designed to be cost effective compared to existing extraction methods, which may require large quantities of consumables, produce substantial volumes of organic waste, or require costly instrumentation or equipment. Reagent grade sand, soil collected from the native grassland in proximity to Eastern Washington University (EWU), and sediment samples collected from the Spokane river were used as sample matrices during method development. These matrices were fortified with eight AWIs of varying chemical properties that are representative of a variety of household, industrial, and agricultural sources. The recoveries of the AWIs spiked onto these matrices were determined in the extracts using gas chromatography/mass spectrometry (GC/MS). These values reflect the efficiency of the method for extraction of these analytes from representative environmental matrices. Recoveries ranged from 46.1% to 110% in the fortified soil and from 49.2% to 118.6% in the fortified sediment samples, which is comparable with existing methods for the study analytes. The optimized method was then used to quantify AWIs in a biosolid-amended soil. Indole and p-cresol were detected in the biosolid-amended soil.
本研究的目的是开发、优化并展示一种基于超声辅助萃取（UAE）的方法，用于从土壤和沉积物样品中萃取具有一系列物理化学性质的有机人为废物指标（AWIs）。与现有萃取方法相比，优化后的方法设计得具有成本效益，现有方法可能需要大量耗材、产生大量有机废物，或者需要昂贵的仪器设备。在方法开发过程中，使用试剂级沙子、从东华盛顿大学（EWU）附近的原生草原采集的土壤以及从斯波坎河采集的沉积物样品作为样品基质。这些基质添加了八种化学性质各异的AWIs，它们代表了各种家庭、工业和农业来源。使用气相色谱/质谱联用仪（GC/MS）测定提取物中添加到这些基质上的AWIs的回收率。这些值反映了该方法从代表性环境基质中萃取这些分析物的效率。在强化土壤中，回收率范围为46.1%至110%，在强化沉积物样品中为49.2%至118.6%，这与研究分析物的现有方法相当。然后使用优化后的方法对生物固体改良土壤中的AWIs进行定量。在生物固体改良土壤中检测到了吲哚和对甲酚。
