Navarro Patricia, Etxebarria Nestor, Arana Gorka
Department of Analytical Chemistry, Science and Technology Faculty, University of the Basque Country, 48080 Bilbao, Spain.
Anal Chim Acta. 2009 Aug 26;648(2):178-82. doi: 10.1016/j.aca.2009.06.062. Epub 2009 Jul 3.
Focused ultrasonic-assisted extraction (FUSE) is a new and particular technique based on the cavitation effect. In this work, the focused ultrasound assisted extraction was studied and developed for the extraction of polycyclic aromatic hydrocarbons from marine sediments and mussel tissues. The variables influencing the extraction (amplitude of the ultrasound pulse, the extraction time and the solvent) were studied by a full factorial design and a central composite design. As a result, flat response surfaces were obtained and the most convenient conditions were 45% of ultrasound amplitude, 120s of extraction time and 5 mL of acetone. Both accuracy and precision of the method were evaluated by means of two certified reference materials (marine sediment and mussel tissue) and the results were also compared to those obtained by microwave assisted extraction.
聚焦超声辅助萃取（FUSE）是一种基于空化效应的新型特殊技术。在本研究中，对聚焦超声辅助萃取技术进行了研究与开发，用于从海洋沉积物和贻贝组织中萃取多环芳烃。通过全因子设计和中心复合设计研究了影响萃取的变量（超声脉冲幅度、萃取时间和溶剂）。结果得到了平缓的响应面，最适宜的条件为超声幅度45%、萃取时间120秒和5毫升丙酮。通过两种有证标准物质（海洋沉积物和贻贝组织）对该方法的准确度和精密度进行了评估，并将结果与微波辅助萃取法所得结果进行了比较。
