Hains Peter G, Truscott Roger J W
Save Sight Institute, University of Sydney, Sydney, NSW, 2001, Australia.
J Proteome Res. 2007 Oct;6(10):3935-43. doi: 10.1021/pr070138h. Epub 2007 Sep 7.
The urea-soluble proteins from the nucleus of two young, two aged, and two early-stage nuclear cataract lenses were subjected to tryptic digestion and analysis by 2D LC-MS/MS. Several novel post-translational modifications were identified. Deamidation was, by far, the most common modification. A number of differences were found in cataract compared to normal lenses, most notably an increase in the number of oxidized tryptophan residues. Semiquantitative analysis revealed that there appeared to be a trend toward increased levels of deamidation with age; however, there was no apparent increase upon the onset of nuclear cataract. This is in contrast to Trp oxidation, where an increase in the extent of modification was apparent in cataract lenses when compared to aged normal lenses. These findings suggest Trp oxidation may be involved in nuclear cataract development.
对来自两枚年轻晶状体、两枚老年晶状体以及两枚早期核性白内障晶状体细胞核的尿素可溶性蛋白质进行胰蛋白酶消化，并通过二维液相色谱-串联质谱法（2D LC-MS/MS）进行分析。鉴定出了几种新的翻译后修饰。到目前为止，脱酰胺作用是最常见的修饰。与正常晶状体相比，在白内障中发现了许多差异，最显著的是氧化色氨酸残基数量的增加。半定量分析显示，脱酰胺水平似乎有随年龄增加的趋势；然而，在核性白内障发病时并没有明显增加。这与色氨酸氧化形成对比，与老年正常晶状体相比，白内障晶状体中修饰程度的增加很明显。这些发现表明色氨酸氧化可能参与核性白内障的发展。
