Sano Ken-Ichi, Yoshii Shigeo, Yamashita Ichiro, Shiba Kiyotaka
Department of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo 135-8550, Japan.
Nano Lett. 2007 Oct;7(10):3200-2. doi: 10.1021/nl071921b. Epub 2007 Sep 7.
Ferritin nanoparticles ornamented with a Ti-binding peptide are versatile nanoscaled building blocks. Their specific binding ability is strong enough to position them on nanopatterned Ti regions on a Pt substrate. Furthermore, the peptides mineralization activity enables the formation of titania on the outer side of the particle, and the particle's inner nanospaces can serve as a carrier for inorganic nanodots. Making use of all these properties, here we show controlled in aqua fabrication of three-dimensional nanoscale structures. The X-Y positioning obeyed the specific binding of the peptide, while fabrication in the Z-dimension entailed stepwise formation of titania and ferritin layers by alternately applying the binding and mineralization abilities of the Ti-binding peptide. This method paves the way for in aqua fabrication of nanodevices having complicated structures and functions.
装饰有钛结合肽的铁蛋白纳米颗粒是多功能的纳米级构建块。它们的特异性结合能力很强，足以将它们定位在铂基底上的纳米图案化钛区域上。此外，肽的矿化活性能够在颗粒外侧形成二氧化钛，并且颗粒内部的纳米空间可以作为无机纳米点的载体。利用所有这些特性，我们在此展示了在水中可控制造三维纳米级结构。X-Y定位遵循肽的特异性结合，而Z维度的制造则通过交替应用钛结合肽的结合和矿化能力逐步形成二氧化钛层和铁蛋白层。这种方法为在水中制造具有复杂结构和功能的纳米器件铺平了道路。
