Liu Li, Jiang Hui-qing, Zhang Xiao-lan, Zhao Dong-qiang, Song Mei
Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):801-3.
To study the effect of Arg-Gly-Asp-Ser (RGDS) tetrapeptide on the expression of matrix metalloproteinase-13 (MMP-13) and the tissue inhibitor of metalloproteinase-1(TIMP-1) in hepatic stellate cell(HSC) stimulated by fibronectin (FN).
The expression of MMP-13 was evaluated by RT-PCR. TIMP-1 was analyzed by in-situ hybridization at mRNA level and by Western blot at protein level.
Treated by RGDS tetrapeptide for 2 h, the mRNA expression of MMP-13 was upregulated but the expression of TIMP-1 mRNA and its protein in HSC was inhibited.
The expression of MMP-13 can be induced by RGDS tetrapeptide and the expression of TIMP-1 can be inhibited by RGDS tetrapeptide, which is one of the mechanisms of its anti-fibrogenesis.
研究精氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）四肽对纤连蛋白（FN）刺激的肝星状细胞（HSC）中基质金属蛋白酶-13（MMP-13）和金属蛋白酶组织抑制剂-1（TIMP-1）表达的影响。
采用逆转录-聚合酶链反应（RT-PCR）评估MMP-13的表达。通过原位杂交在mRNA水平分析TIMP-1，并在蛋白质水平通过蛋白质免疫印迹法进行分析。
用RGDS四肽处理2小时后，HSC中MMP-13的mRNA表达上调，但TIMP-1 mRNA及其蛋白的表达受到抑制。
RGDS四肽可诱导MMP-13的表达，抑制TIMP-1的表达，这是其抗纤维化作用机制之一。
