Lee Hyun Kyoung, Seo In Ae, Seo Eunhui, Seo Su-Yeong, Lee Hye Jeong, Park Hwan Tae
Department of Physiology, College of Medicine, Dong-A University, 3-1, Dongdaesin-Dong, Seo-Gu, Busan 602-714, South Korea.
Biochem Biophys Res Commun. 2007 Nov 3;362(4):1057-62. doi: 10.1016/j.bbrc.2007.08.143. Epub 2007 Aug 31.
Netrin and its receptors, DCC (Deleted in Colorectal Cancer) and Unc5, are proposed to be involved in the axon guidance and neuroglial migration during development. However, accumulating evidence implies that they may also participate in the cell survival and apoptosis. Here, we show that netrin-1 induces proliferation of Schwann cells. Unc5b is the sole receptor expressed in RT4 schwannoma cells and adult primary Schwann cells, and netrin-1 and Unc5b are found to be expressed in the injured sciatic nerve. It was also found that the netrin-1-induced Schwann cell proliferation was blocked by the specific inhibition of Unc5b expression with RNAi. These data suggest that netrin-1 could be an endogenous trophic factor for Schwann cells in the injured peripheral nerves.
据推测，Netrin及其受体DCC（结直肠癌缺失基因）和Unc5在发育过程中参与轴突导向和神经胶质迁移。然而，越来越多的证据表明它们也可能参与细胞存活和凋亡。在此，我们表明Netrin-1可诱导雪旺细胞增殖。Unc5b是RT4雪旺细胞瘤细胞和成年原代雪旺细胞中唯一表达的受体，并且发现Netrin-1和Unc5b在受损的坐骨神经中表达。还发现通过RNAi特异性抑制Unc5b表达可阻断Netrin-1诱导的雪旺细胞增殖。这些数据表明，Netrin-1可能是受损周围神经中雪旺细胞的内源性营养因子。
