Salehi Majid, Naseri-Nosar Mahdi, Ebrahimi-Barough Somayeh, Nourani Mohammdreza, Vaez Ahmad, Farzamfar Saeed, Ai Jafar
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 1417755469, Tehran, Iran.
Nano Biotechnology Research Center, Baqiyatallah University of Medical Sciences, 1435944711, Tehran, Iran.
J Physiol Sci. 2018 Sep;68(5):579-587. doi: 10.1007/s12576-017-0564-6. Epub 2017 Sep 6.
The current study aimed to enhance the efficacy of peripheral nerve regeneration using a hydroxyapatite nanoparticle-containing collagen type I hydrogel. A solution of type I collagen, extracted from the rat tails, was incorporated with hydroxyapatite nanoparticles (with the average diameter of ~212 nm) and crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) to prepare the hydrogel. The Schwann cell cultivation on the prepared hydrogel demonstrated a significantly higher cell proliferation than the tissue culture plate, as positive control, after 48 h (n = 3, P < 0.005) and 72 h (n = 3, P < 0.01). For in vivo evaluation, the prepared hydrogel was administrated on the sciatic nerve crush injury in Wistar rats. Four groups were studied: negative control (with injury but without interventions), positive control (without injury), collagen hydrogel and hydroxyapatite nanoparticle-containing collagen hydrogel. After 12 weeks, the administration of hydroxyapatite nanoparticle-containing collagen significantly (n = 4, P < 0.005) enhanced the functional behavior of the rats compared with the collagen hydrogel and negative control groups as evidenced by the sciatic functional index, hot plate latency and compound muscle action potential amplitude measurements. The overall results demonstrated the applicability of the produced hydrogel for the regeneration of peripheral nerve injuries.
本研究旨在使用含羟基磷灰石纳米颗粒的I型胶原水凝胶提高周围神经再生的疗效。从大鼠尾巴中提取的I型胶原溶液与羟基磷灰石纳米颗粒（平均直径约为212nm）混合，并与1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺（EDC）交联以制备水凝胶。在制备的水凝胶上培养雪旺细胞，48小时（n = 3，P < 0.005）和72小时（n = 3，P < 0.01）后，与作为阳性对照的组织培养板相比，细胞增殖显著更高。为了进行体内评估，将制备的水凝胶应用于Wistar大鼠的坐骨神经挤压伤。研究了四组：阴性对照（有损伤但无干预）、阳性对照（无损伤）、胶原水凝胶和含羟基磷灰石纳米颗粒的胶原水凝胶。12周后，与胶原水凝胶组和阴性对照组相比，含羟基磷灰石纳米颗粒的胶原给药显著（n = 4，P < 0.005）增强了大鼠的功能行为，坐骨神经功能指数、热板潜伏期和复合肌肉动作电位幅度测量结果证明了这一点。总体结果表明所制备的水凝胶适用于周围神经损伤的再生。
