Travis Emma R, Bruce Neil C, Rosser Susan J
Institute of Biomedical and Life Sciences, University of Glasgow, Bower Building, Glasgow, UK.
Environ Pollut. 2008 May;153(1):119-26. doi: 10.1016/j.envpol.2007.07.015. Epub 2007 Sep 6.
The contamination of the environment with explosive residues presents a serious ecological problem at sites across the world, with the highly toxic compound trinitrotoluene (TNT) the most widespread contaminant. This study examines the soil microbial community composition across a long-term TNT-contaminated site. It also investigates the extent of nitroaromatic contamination and its effect on vegetation. Concentrations of TNT and its metabolites varied across the site and this was observed to dramatically impact on the extent and diversity of the vegetation, with the most heavily contaminated area completely devoid of vegetation. Bryophytes were seen to be particularly sensitive to TNT contamination. The microbial population experienced both a reduction in culturable bacterial numbers and a shift in composition at the high concentrations of TNT. DGGE and community-level physiological profiling (CLPP) revealed a clear change in both the genetic and functional diversity of the soil when soil was contaminated with TNT.
爆炸残留物对环境的污染在全球各地的场地都构成了严重的生态问题，其中剧毒化合物三硝基甲苯（TNT）是分布最广的污染物。本研究调查了一个长期受TNT污染场地的土壤微生物群落组成。它还研究了硝基芳烃污染的程度及其对植被的影响。TNT及其代谢物的浓度在整个场地有所不同，并且观察到这对植被的范围和多样性产生了巨大影响，污染最严重的区域完全没有植被。苔藓植物被认为对TNT污染特别敏感。在高浓度TNT条件下，微生物种群的可培养细菌数量减少且组成发生了变化。变性梯度凝胶电泳（DGGE）和群落水平生理图谱分析（CLPP）显示，当土壤被TNT污染时，土壤的遗传和功能多样性都发生了明显变化。
