用于分离人呼吸道合胞病毒（HRSV）的HeLa-I、HEp-2和NCI-H292细胞系的比较。

Comparison of HeLa-I, HEp-2 and NCI-H292 cell lines for the isolation of human respiratory syncytial virus (HRSV).

作者信息

Perini Ana Priscila, Barbosa Maria Luisa, Botosso Viviane Fongaro, de Moraes Claudia Trigo Pedroso, Gillio Alfredo E, Hens Noeli, Stewien Klaus E, Durigon Edison L

机构信息

Universidade de São Paulo, Instituto de Ciências Biomédicas, São Paulo, Brazil.

出版信息

J Virol Methods. 2007 Dec;146(1-2):368-71. doi: 10.1016/j.jviromet.2007.07.004. Epub 2007 Sep 7.

DOI:10.1016/j.jviromet.2007.07.004

PMID:17825929

Abstract

Generally, laboratory diagnosis of viral respiratory infections utilizes virus isolation in cell culture and immunofluorescence assays. In this study, three cell lines (HEp-2, NCI-H292 and HeLa-I) were used for HRSV isolation of strains obtained from patients admitted at HU-USP with respiratory tract disease. HRSV was isolated in 46% (37) of 80 specimens inoculated in HeLa-I, 48% (39) in HEp-2, and 36.3% (29) in NCI-H292. Immunofluorescence was considered the gold standard and yielded 53% positive (43). The results from both methods combined had better sensitivity (73.2%) compared to either method alone. Comparing results between the cell lines with HEp-2 cells as the benchmark, the greatest sensitivity (72.2%) was observed in HeLa-I. This data shows that HeLa-I is adequate for HRSV isolation, giving results similar to the HEp-2 cells. The combined use of the HEp-2, HeLa-I and NCI-H292 cells improve the detection of HRSV.

摘要

一般来说，病毒性呼吸道感染的实验室诊断采用细胞培养中的病毒分离和免疫荧光检测。在本研究中，使用了三种细胞系（HEp-2、NCI-H292和HeLa-I）对从圣保罗大学医院因呼吸道疾病入院的患者中分离出的毒株进行人呼吸道合胞病毒（HRSV）分离。接种于HeLa-I的80份标本中有46%（37份）分离出HRSV，接种于HEp-2的有48%（39份），接种于NCI-H292的有36.3%（29份）。免疫荧光被视为金标准，阳性率为53%（43份）。两种方法结合的结果比单独使用任何一种方法具有更高的敏感性（73.2%）。以HEp-2细胞为基准比较各细胞系的结果，HeLa-I的敏感性最高（72.2%）。该数据表明HeLa-I适合用于HRSV分离，其结果与HEp-2细胞相似。联合使用HEp-2、HeLa-I和NCI-H292细胞可提高HRSV的检测率。