Azevedo Luciana G, Muccillo-Baisch Ana L, Filgueira Daza de M V B, Boyle Robert T, Ramos Daniela F, Soares Andrea D, Lerner Clea, Silva Pedro A, Trindade Gilma S
Departamento de Ciências Fisiológicas, Fundação Universidade Federal do Rio Grande (FURG), Rio Grande, (96201-900), Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2008 Jan;147(1):36-42. doi: 10.1016/j.cbpc.2007.07.007. Epub 2007 Aug 1.
Three crude extracts of Aplysina caissara, a marine sponge endemic to Brazil, were tested against a hepatoma cell line and Mycobacterium tuberculosis. The results demonstrate that all extracts are toxic and capable of inhibiting cellular growth. Additionally, the extracts produced morphological aberrations and inhibited cell attachment to culture substrates. These effects were dose/time dependent. Our results also suggest that reactive oxygen species (ROS) production is not involved in the cytotoxic processes levied by the extracts employed in this study and that active metabolites are likely to be present in the polar fractions of the crude extracts. Finally, our results indicate that all three extracts exhibit a moderate anti-tuberculosis capacity, and that the removal of an extract's lipid fraction appears to diminish this activity.
对巴西特有的海洋海绵卡西拉阿普lysina的三种粗提物进行了针对肝癌细胞系和结核分枝杆菌的测试。结果表明，所有提取物均具有毒性且能够抑制细胞生长。此外，提取物还产生形态畸变并抑制细胞附着于培养底物。这些作用呈剂量/时间依赖性。我们的结果还表明，活性氧（ROS）的产生与本研究中所用提取物引起的细胞毒性过程无关，并且活性代谢物可能存在于粗提物的极性部分中。最后，我们的结果表明，所有三种提取物均表现出中等的抗结核能力，并且去除提取物的脂质部分似乎会降低这种活性。
