Department of Molecular and Cellular Biology, Institute of Biology, Federal Fluminense University, Niteroi, 24020-141, RJ, Brazil.
Department of Marine Biology, Institute of Biology, Federal Fluminense University, Niteroi, 24020-141, RJ, Brazil.
Mar Drugs. 2011;9(8):1346-1358. doi: 10.3390/md9081346. Epub 2011 Aug 10.
The ischemic disorders, in which platelet aggregation and blood coagulation are involved, represent a major cause of disability and death worldwide. The antithrombotic therapy has unsatisfactory performance and may produce side effects. So, there is a need to seek molecules with antithrombotic properties. Marine organisms produce substances with different well defined ecological functions. Moreover, some of these molecules also exhibit pharmacological properties such as antiviral, anticancer, antiophidic and anticoagulant properties. The aim of this study was to evaluate, through in vitro tests, the effect of two extracts of brown algae and ten marine sponges from Brazil on platelet aggregation and blood coagulation. Our results revealed that most of the extracts were capable of inhibiting platelet aggregation and clotting measured by plasma recalcification tests, prothrombin time, activated partial thromboplastin time, and fibrinogenolytic activity. On the other hand, five of ten species of sponges induced platelet aggregation. Thus, the marine organisms studied here may have molecules with antithrombotic properties, presenting biotechnological potential to antithrombotic therapy. Further chemical investigation should be conducted on the active species to discover useful molecules for the development of new drugs to treat clotting disorders.
缺血性疾病涉及血小板聚集和血液凝固，是全球残疾和死亡的主要原因。抗血栓治疗效果不理想，可能产生副作用。因此，需要寻找具有抗血栓特性的分子。海洋生物产生具有不同明确生态功能的物质。此外，这些分子中的一些还表现出药理特性，如抗病毒、抗癌、抗蛇毒和抗凝特性。本研究的目的是通过体外试验评估来自巴西的两种褐藻提取物和十种海洋海绵提取物对血小板聚集和血液凝固的影响。我们的结果表明，大多数提取物能够抑制由血浆再钙化试验、凝血酶原时间、活化部分凝血活酶时间和纤维蛋白原溶解活性测量的血小板聚集和凝血。另一方面，十种海绵中有五种诱导血小板聚集。因此，这里研究的海洋生物可能具有抗血栓特性的分子，为抗血栓治疗提供生物技术潜力。应对活性物种进行进一步的化学研究，以发现用于开发治疗凝血障碍的新药的有用分子。
