Walsh Lance P, Anderson J Kyle, Baker Meredith R, Han Bumsoo, Hsieh Jer-Tsong, Lotan Yair, Cadeddu Jeffrey A
Department of Urology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9110, USA.
Urology. 2007 Aug;70(2):380-4. doi: 10.1016/j.urology.2007.03.007.
To define the temperature-time points that result in cell death in a human renal cell carcinoma cell (RCC) line in vitro.
Cellular viability and clonogenic cell survival were determined for human A498 RCC cells after thermal treatment. Various temperature (45 degrees C to 70 degrees C) and time (1 to 30 minutes) combinations were used. Cell viability was assessed by vital dye uptake and clonogenic cell survival. Mathematical Arrhenius modeling was performed to construct a graphic display of A498 cell thermal sensitivity.
Temperature-time points at which 99% or greater cell death occurred according to the vital dye assay were 55 degrees C for 30 minutes, 60 degrees C for 10 minutes, and 65 degrees C for 8 minutes. Clonogenic survival studies confirmed that cells treated at these temperature-time points failed to grow even after 10 days.
These in vitro results show that short exposure to temperatures higher than 70 degrees C is lethal in the A498 RCC cell line. Lower temperatures in the 60 degrees C range require more prolonged heating to cause cell death. Knowledge of these temperatures will be useful to better plan and monitor complex radiofrequency ablations.
确定体外培养的人肾癌细胞系中导致细胞死亡的温度-时间点。
对人A498肾癌细胞进行热处理后，测定细胞活力和克隆形成细胞存活率。采用了各种温度（45℃至70℃）和时间（1至30分钟）的组合。通过活细胞染料摄取和克隆形成细胞存活率评估细胞活力。进行数学阿伦尼乌斯模型构建以绘制A498细胞热敏感性的图形展示。
根据活细胞染料测定法，导致99%或更高细胞死亡的温度-时间点为55℃处理30分钟、60℃处理10分钟和65℃处理8分钟。克隆形成存活研究证实，在这些温度-时间点处理的细胞即使在10天后也无法生长。
这些体外研究结果表明，短时间暴露于高于70℃的温度对A498肾癌细胞系具有致死性。60℃范围内的较低温度需要更长时间的加热才能导致细胞死亡。了解这些温度将有助于更好地规划和监测复杂的射频消融术。
