缺氧调节足月人原代滋养层细胞中脂肪酸结合蛋白的表达。
Hypoxia regulates the expression of fatty acid-binding proteins in primary term human trophoblasts.
作者信息
Biron-Shental Tal, Schaiff W Timothy, Ratajczak Christine K, Bildirici Ibrahim, Nelson D Michael, Sadovsky Yoel
机构信息
Department of Obstetrics and Gynecology, Washington University in St Louis School of Medicine, St Louis, MO, USA.
出版信息
Am J Obstet Gynecol. 2007 Nov;197(5):516.e1-6. doi: 10.1016/j.ajog.2007.03.066. Epub 2007 Sep 10.
OBJECTIVE
Fatty acids (FAs) are essential for fetal development. Cellular FA uptake is modulated by fatty acid-binding proteins (FABPs). We hypothesized that hypoxia regulates the expression of FABPs in human trophoblasts.
STUDY DESIGN
Primary term human trophoblasts were cultured for 72 hours in either standard (O2 = 20%) or hypoxic (O2 < 1%) conditions. FABP expression was interrogated using polymerase chain reaction and Western immunoblotting. Trophoblast lipid droplets were examined using dipyrromethene boron difluoride 493/503 staining.
RESULTS
We detected the expression of FABP1, -3, -4, -5, and pm but not FABP2 or FABP6-9 subtypes in trophoblasts. Exposure to hypoxia markedly increased lipid droplet accumulation in trophoblasts. Consistent with this observation, hypoxia enhanced the expression of FABP1, -3, and -4. Lastly, agonists of peroxisome proliferator-activated receptor-gamma enhanced the expression of FABP1 and -4 in trophoblasts.
CONCLUSION
Hypoxia enhances the expression of FABP1, -3, and -4 in term human trophoblasts, suggesting that FABPs support fat accumulation in the hypoxic placenta.
目的
脂肪酸（FAs）对胎儿发育至关重要。细胞脂肪酸摄取受脂肪酸结合蛋白（FABPs）调节。我们推测缺氧可调节人滋养层细胞中FABPs的表达。
研究设计
将足月人原代滋养层细胞在标准（氧气浓度 = 20%）或缺氧（氧气浓度 < 1%）条件下培养72小时。使用聚合酶链反应和Western免疫印迹法检测FABP的表达。使用二吡咯亚甲基硼二氟化物493/503染色检查滋养层细胞脂滴。
结果
我们在滋养层细胞中检测到FABP1、-3、-4、-5和pm的表达，但未检测到FABP2或FABP6 - 9亚型的表达。暴露于缺氧环境显著增加了滋养层细胞中脂滴的积累。与此观察结果一致，缺氧增强了FABP1、-3和-4的表达。最后，过氧化物酶体增殖物激活受体γ激动剂增强了滋养层细胞中FABP1和-4的表达。
结论
缺氧增强了足月人滋养层细胞中FABP1、-3和-4的表达，表明FABPs支持缺氧胎盘中的脂肪积累。
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