Lin Yi-Ruu, Chen Hwei-Hsien, Ko Chien-Hsin, Chan Ming-Huan
Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
Life Sci. 2007 Sep 8;81(13):1071-8. doi: 10.1016/j.lfs.2007.08.014. Epub 2007 Aug 19.
The antinociceptive actions of honokiol and magnolol, two major bioactive constituents of the bark of Magnolia officinalis, were evaluated using tail-flick, hot-plate and formalin tests in mice. The effects of honokiol and magnolol on the formalin-induced c-Fos expression in the spinal cord dorsal horn as well as motor coordination and cognitive function were examined. Data showed that honokiol and magnolol did not produce analgesia in tail-flick, hot-plate paw-shaking and neurogenic phase of the overt nociception induced by intraplantar injection of formalin. However, honokiol and magnolol reduced the inflammatory phase of formalin-induced licking response. Consistently, honokiol and magnolol significantly decreased formalin-induced c-Fos protein expression in superficial (I-II) laminae of the L4-L5 lumbar dorsal horn. However, honokiol and magnolol did not elicit motor incoordination and memory dysfunction at doses higher than the analgesic dose. These results demonstrate that honokiol and magnolol effectively alleviate the formalin-induced inflammatory pain without motor and cognitive side effects, suggesting their therapeutic potential in the treatment of inflammatory pain.
厚朴酚与和厚朴酚是厚朴树皮中的两种主要生物活性成分，利用小鼠甩尾试验、热板试验和福尔马林试验对它们的抗伤害感受作用进行了评估。研究了厚朴酚与和厚朴酚对福尔马林诱导的脊髓背角c-Fos表达以及运动协调性和认知功能的影响。数据表明，厚朴酚与和厚朴酚在甩尾试验、热板试验、爪部抖动试验以及足底注射福尔马林诱导的明显伤害感受的神经源性期均未产生镇痛作用。然而，厚朴酚与和厚朴酚可减轻福尔马林诱导的舔舐反应的炎症期。同样，厚朴酚与和厚朴酚显著降低了L4-L5腰段脊髓背角浅层（I-II层）福尔马林诱导的c-Fos蛋白表达。然而，厚朴酚与和厚朴酚在高于镇痛剂量时并未引起运动不协调和记忆功能障碍。这些结果表明，厚朴酚与和厚朴酚可有效减轻福尔马林诱导的炎性疼痛，且无运动和认知方面的副作用，提示它们在治疗炎性疼痛方面具有潜在的治疗价值。
