Patra A K, Le Roux X, Grayston S J, Loiseau P, Louault F
Université de Lyon, Université Lyon 1, UMR 5557 Ecologie Microbienne, CNRS-Université Lyon 1, USC 1193 INRA, IFR41, 43 Boulevard du 11 novembre 1918, 69622 Villeurbanne, France.
Bioresour Technol. 2008 Jun;99(9):3545-51. doi: 10.1016/j.biortech.2007.07.051. Epub 2007 Sep 10.
In a field experiment we have examined the effect of long-term grassland management regimes (viz., intensive versus extensive) and dominant plant species (viz., Arrhenatherum elatius, Holcus lanatus and Dactylis glomerata) on soil organic carbon (SOC) build up, soil microbial communities using biomarker phospholipid fatty acids (PLFA), and the relationship between SOC and PLFAs of major groups of microorganisms (viz., bacteria, fungi, and actinomycetes). The results have revealed that changes in SOC were not significantly affected by the intensity of management or by the plant species composition or by their interaction. The amount of PLFA of each microbial group was affected weakly by management regime and plant species, but the canonical variance analysis (CVA), based on individual PLFA values, demonstrated significant (P<0.05) effects of management regime and plant species on the composition of microbial community. Positive and significant (P<0.01) relationships were observed between PLFA of bacteria (R2=0.47), fungi (R2=0.33), actinomycetes (R2=0.71) and total microbial PLFA (R2=0.53) and SOC content.
在一项田间试验中，我们研究了长期草地管理方式（即集约型与粗放型）和优势植物物种（即燕麦草、绒毛草和鸭茅）对土壤有机碳（SOC）积累、利用生物标志物磷脂脂肪酸（PLFA）分析的土壤微生物群落，以及主要微生物类群（即细菌、真菌和放线菌）的SOC与PLFA之间关系的影响。结果表明，SOC的变化不受管理强度、植物物种组成或它们之间相互作用的显著影响。各微生物类群的PLFA含量受管理方式和植物物种的影响较弱，但基于个体PLFA值的典型变量分析（CVA）表明，管理方式和植物物种对微生物群落组成具有显著（P<0.05）影响。观察到细菌（R2=0.47）、真菌（R2=0.33）、放线菌（R2=0.71）和总微生物PLFA（R2=0.53）的PLFA与SOC含量之间存在显著正相关（P<0.01）关系。
