Chen Chun-Yen, Lu Wei-Bin, Liu Chien-Hung, Chang Jo-Shu
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Bioresour Technol. 2008 Jun;99(9):3609-16. doi: 10.1016/j.biortech.2007.07.037. Epub 2007 Sep 10.
An indigenous purple nonsulfur bacterium Rhodopseudomonas palustris WP3-5 was used to produce hydrogen phototrophically from acetate (HAc) and butyrate (HBu), which are the major soluble products from acidogenic dark fermentation. Statistical experimental design methodology was applied to identify optimal composition of the two carbon substrates in the medium, leading to better H2 production performance of R. palustris WP3-5. Three performance indexes were used to assess the effectiveness of the phototrophic H2 production; they were H2 yield (Y H2), maximum H2 production rate (Rmax) and maximum cumulative H2 evolution (Hmax). An overlay contour plot was used to determine the optimal concentration range of HAc and HBu, taking into account all three performance indexes (i.e., Rmax, Hmax, and Y H2) simultaneously. With the response surface analysis, R. palustris WP3-5 could produce H2 efficiently with the best Rmax, Hmax, and Y H2 of 39.5 ml/h, 2738 ml, and 51.6%, respectively. This performance is superior to most reported values in the literature, indicating that the statistical experimental design is an effective tool to improve phototrophic H2 production with R. palustris WP3-5.
一种本地紫色非硫细菌沼泽红假单胞菌WP3-5被用于从醋酸盐（HAc）和丁酸盐（HBu）进行光养产氢，醋酸盐和丁酸盐是产酸型暗发酵的主要可溶性产物。应用统计实验设计方法来确定培养基中两种碳底物的最佳组成，从而使沼泽红假单胞菌WP3-5具有更好的产氢性能。使用三个性能指标来评估光养产氢的有效性；它们是氢气产率（Y H2）、最大氢气产生速率（Rmax）和最大累积氢气释放量（Hmax）。考虑到所有三个性能指标（即Rmax、Hmax和Y H2），使用叠加等高线图来确定HAc和HBu的最佳浓度范围。通过响应面分析，沼泽红假单胞菌WP3-5能够高效产氢，其最佳的Rmax、Hmax和Y H2分别为39.5 ml/h、2738 ml和51.6%。这一性能优于文献中报道的大多数值，表明统计实验设计是提高沼泽红假单胞菌WP3-5光养产氢的有效工具。
