Liu Bing-Feng, Ren Nan-Qi, Xing De-Feng, Ding Jie, Zheng Guo-Xiang, Guo Wan-Qian, Xu Ji-Fei, Xie Guo-Jun
State Key Lab of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, No. 202 Haihe Road, Harbin, China.
Bioresour Technol. 2009 May;100(10):2719-23. doi: 10.1016/j.biortech.2008.12.020. Epub 2009 Feb 5.
In order to increase the hydrogen yield from glucose, hydrogen production by immobilized Rhodopseudomonas faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria Ethanoligenens harbinense B49 was investigated. The soluble metabolites from dark-fermentation mainly were ethanol and acetate, which could be further utilized for photo-hydrogen production. Hydrogen production by B49 was noticeably affected by the glucose and phosphate buffer concentration. The maximum hydrogen yield (1.83 mol H(2)/mol glucose) was obtained at 9 g/l glucose. In addition, we found that the ratio of acetate/ethanol (A/E) increased with increasing phosphate buffer concentration, which is favorable to further photo-hydrogen production. The total hydrogen yield during dark- and photo-fermentation reached its maximum value (6.32 mol H(2)/mol glucose) using 9 g/l glucose, 30 mmol/l phosphate buffers and immobilized R. faecalis RLD-53. Results demonstrated that the combination of dark- and photo- fermentation was an effective and efficient process to improve hydrogen yield from a single substrate.
为了提高葡萄糖的产氢量，研究了利用哈尔滨产乙醇杆菌B49乙醇发酵细菌的可溶性代谢产物，通过固定化粪红假单胞菌RLD-53产氢。黑暗发酵产生的可溶性代谢产物主要是乙醇和乙酸盐，它们可进一步用于光催化产氢。B49的产氢受到葡萄糖和磷酸盐缓冲液浓度的显著影响。在葡萄糖浓度为9 g/l时，获得了最大产氢量（1.83 mol H₂/mol葡萄糖）。此外，我们发现乙酸盐/乙醇（A/E）的比例随着磷酸盐缓冲液浓度的增加而增加，这有利于进一步光催化产氢。使用浓度为9 g/l的葡萄糖、30 mmol/l的磷酸盐缓冲液和固定化的粪红假单胞菌RLD-53，黑暗发酵和光发酵过程中的总产氢量达到最大值（6.32 mol H₂/mol葡萄糖）。结果表明，黑暗发酵和光发酵相结合是提高单一底物产氢量的有效且高效的方法
