Jih Charng-Gwo, Huang Ju-Sheng, Lin Huei-Jen, Chou Hsin-Hsien
Department of Environmental Resources Management, Tajen University, Pingtung County 907, Taiwan, ROC.
Bioresour Technol. 2008 Jun;99(9):3484-90. doi: 10.1016/j.biortech.2007.07.056. Epub 2007 Sep 10.
A batch feed study using nitrifiers that had been continuously acclimated under a low-ammonia environment showed that a sudden change of ammonia concentration resulted in sluggish physiological adaptation and biochemical reaction of nitrifiers (i.e., indicated by the parameter specific oxygen utilization rate). When the one-stage continuous-stirred tank reactor (CSTR) system was maintained at a short microbial cell residence time and a high volumetric loading rate, an accumulation of nitrite occurred. Under such circumstances, ammonia and nitrite oxidation both limit overall nitrification at different stages of the process. Batch studies with biomass respectively removed from the front and rear reactors (i.e., high-ammonia and low-ammonia growth environments) of a two-stage CSTR system showed that the estimated kinetic parameters for nitrifiers with the low-ammonia growth environment were 0.3-0.8-fold lower than those for nitrifiers with the high-ammonia growth environment, possibly leading to inaccurate model simulation results. Accordingly, biomass removed from a CSTR system that had been operated continuously to grow bacteria under a high-substrate environment should be loaded into the batch reactor if the batch reactor method is to be used to estimate kinetic parameters.
一项使用在低氨环境下持续驯化的硝化菌进行的分批进料研究表明，氨浓度的突然变化会导致硝化菌的生理适应和生化反应迟缓（即通过特定氧利用率参数来表明）。当单级连续搅拌釜式反应器（CSTR）系统保持较短的微生物细胞停留时间和较高的体积负荷率时，会发生亚硝酸盐积累。在这种情况下，氨和亚硝酸盐氧化在该过程的不同阶段都会限制整体硝化作用。对分别从两级CSTR系统的前部和后部反应器（即高氨和低氨生长环境）中取出的生物质进行的分批研究表明，低氨生长环境下硝化菌的估计动力学参数比高氨生长环境下硝化菌的动力学参数低0.3 - 0.8倍，这可能导致模型模拟结果不准确。因此，如果要使用分批反应器方法来估计动力学参数，应将从在高底物环境下连续运行以培养细菌的CSTR系统中取出的生物质装入分批反应器中。
