Okazaki Shin, Hattori Yoshiyuki, Saeki Kazuhiko
Department of Biological Sciences, Faculty of Science, Nara Women's University, Nara 630- 8506, Japan.
J Bacteriol. 2007 Nov;189(22):8347-52. doi: 10.1128/JB.00788-07. Epub 2007 Sep 7.
The purB and purH mutants of Mesorhizobium loti exhibited purine auxotrophy and nodulation deficiency on Lotus japonicus. In the presence of adenine, only the purH mutant induced nodule formation and the purB mutant produced few infection threads, suggesting that 5-aminoimidazole-4-carboxamide ribonucleotide biosynthesis catalyzed by PurB is required for the establishment of symbiosis.
百脉根中慢生根瘤菌的purB和purH突变体在日本百脉根上表现出嘌呤营养缺陷型和结瘤缺陷。在腺嘌呤存在的情况下,只有purH突变体诱导结瘤形成,而purB突变体产生的侵染线很少,这表明PurB催化的5-氨基咪唑-4-甲酰胺核糖核苷酸生物合成是共生关系建立所必需的。