反刍短杆菌作为地表水驯化反刍动物粪便污染的指示菌。
Methanobrevibacter ruminantium as an indicator of domesticated-ruminant fecal pollution in surface waters.
作者信息
Ufnar Jennifer A, Wang Shiao Y, Ufnar David F, Ellender R D
机构信息
Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, Mississippi 39406, USA.
出版信息
Appl Environ Microbiol. 2007 Nov;73(21):7118-21. doi: 10.1128/AEM.00911-07. Epub 2007 Sep 7.
Abstract
A PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter ruminantium was developed to detect fecal pollution from domesticated ruminants in environmental water samples. The assay produced the expected amplification product only when the reaction mixture contained DNA extracted from M. ruminantium culture, bovine (80%), sheep (100%), and goat (75%) feces, and water samples from a bovine waste lagoon (100%) and a creek contaminated with bovine lagoon waste (100%). The assay appears to be specific and sensitive and can distinguish between domesticated- and nondomesticated-ruminant fecal pollution in environmental samples.
摘要
开发了一种基于聚合酶链反应（PCR）的检测方法（Mrnif），该方法靶向反刍短杆菌的固氮酶还原酶基因（nifH），用于检测环境水样中来自家养反刍动物的粪便污染。只有当反应混合物中含有从反刍短杆菌培养物、牛粪便（80%）、绵羊粪便（100%）和山羊粪便（75%）中提取的DNA，以及来自牛粪便泻湖的水样（100%）和受牛泻湖废物污染的小溪水样（100%）时，该检测方法才会产生预期的扩增产物。该检测方法似乎具有特异性和敏感性，能够区分环境样品中家养反刍动物和非家养反刍动物的粪便污染。
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