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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Banks M A, Porter D W, Pailes W H, Schwegler-Berry D, Martin W G, Castranova V

Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505.

Comp Biochem Physiol B. 1991;100(4):795-9. doi: 10.1016/0305-0491(91)90292-l.

Rat alveolar type I cells were isolated by enzymatic digestion and purified by centrifugal elutriation and specific surface adsorption. 2. The identity of the harvested cells was confirmed using electronic cell sizing and transmission electron microscopy. 3. Purified cell preparations contained 4.6 +/- 2.3 x 10(6) type I cells/rat lung with a purity of 79 +/- 3%. 4. Isolated type I cells exhibited the following characteristics: mean cell volume = 716 +/- 48 microns 3; diameter = 11.1 +/- 0.7 microns; and cell water content = 0.50 +/- 0.03 microliter/10(6) cells. 5. Taurine content of these alveolar type I cells was measured by HPLC. 6. The intracellular taurine concentration of type I cells was 0.14 +/- 0.07 mM, a value close to that of plasma (0.1 mM).

Banks M A, Porter D W, Pailes W H, Schwegler-Berry D, Martin W G, Castranova V

Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505.

Comp Biochem Physiol B. 1991;100(4):795-9. doi: 10.1016/0305-0491(91)90292-l.

Rat alveolar type I cells were isolated by enzymatic digestion and purified by centrifugal elutriation and specific surface adsorption. 2. The identity of the harvested cells was confirmed using electronic cell sizing and transmission electron microscopy. 3. Purified cell preparations contained 4.6 +/- 2.3 x 10(6) type I cells/rat lung with a purity of 79 +/- 3%. 4. Isolated type I cells exhibited the following characteristics: mean cell volume = 716 +/- 48 microns 3; diameter = 11.1 +/- 0.7 microns; and cell water content = 0.50 +/- 0.03 microliter/10(6) cells. 5. Taurine content of these alveolar type I cells was measured by HPLC. 6. The intracellular taurine concentration of type I cells was 0.14 +/- 0.07 mM, a value close to that of plasma (0.1 mM).