Lee Jangwoen, Armstrong Jennifer, Kreuter Kelly, Tromberg Bruce J, Brenner Matthew
Laser Microbeam and Medical Program, Beckman Laser Institute, University of California, Irvine, CA 92612-1475, USA.
Physiol Meas. 2007 Sep;28(9):1057-66. doi: 10.1088/0967-3334/28/9/007. Epub 2007 Aug 21.
The objective of this study is to establish a cyanide toxicity animal model and to investigate the ability of broadband diffuse optical spectroscopy (DOS) to non-invasively monitor physiological changes that occur during the development of cyanide toxicity in a rabbit model. Broadband DOS combines multi-frequency frequency-domain photon migration (FDPM) with time-independent near-infrared spectroscopy (NIRS) to quantitatively measure bulk tissue absorption and scattering spectra between 600 nm and 1000 nm. Serum cyanide concentration and arterial and venous blood gas analysis at pre- and post-cyanide infusion were presented. To investigate the ability of DOS to non-invasively monitor physiologic changes occurring during development of CN toxicity, tissue concentrations of deoxyhemoglobin [Hb-R], oxyhemoglobin [Hb-O2], cytochrome c oxidase oxidized state [CcO_Ox] and reduced state [CcO_Re] were determined from absorption spectra acquired in 'real time' during cyanide infusions (NaCN 6 mg/60 ml normal saline) in six pathogen-free New Zealand white rabbits. During cyanide infusion, in vivo tissue oxygen saturation increased ( approximately 10%). In addition, broadband DOS was able to detect a concurrent increase in [CcO_Re] and decrease in [CcO_Ox]. Changes in tissue scattering properties in all six animals were detected during these events, confirming the need for DOS-based methods over traditional NIR spectroscopy to obtain accurate results.
本研究的目的是建立氰化物中毒动物模型，并研究宽带漫射光谱（DOS）在兔模型中对氰化物中毒发展过程中发生的生理变化进行无创监测的能力。宽带DOS将多频频域光子迁移（FDPM）与非时间分辨近红外光谱（NIRS）相结合，以定量测量600nm至1000nm之间的组织总体吸收和散射光谱。给出了氰化物输注前后的血清氰化物浓度以及动脉和静脉血气分析结果。为了研究DOS对氰化物中毒发展过程中发生的生理变化进行无创监测的能力，在六只无特定病原体的新西兰白兔静脉输注氰化物（6mg NaCN溶于60ml生理盐水中）期间，从“实时”采集的吸收光谱中测定了脱氧血红蛋白[Hb-R]、氧合血红蛋白[Hb-O2]、细胞色素c氧化酶氧化态[CcO_Ox]和还原态[CcO_Re]的组织浓度。在输注氰化物期间，体内组织氧饱和度增加（约10%）。此外，宽带DOS能够检测到[CcO_Re]同时增加和[CcO_Ox]减少。在这些过程中检测到了所有六只动物的组织散射特性变化，这证实了与传统近红外光谱相比，需要基于DOS的方法才能获得准确结果。
