Yoshida Munehiro, Okada Toshihide, Namikawa Yûichi, Matsuzaki Yoshimi, Nishiyama Toshimasa, Fukunaga Kenji
Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty of Engineering, Kansai University, Suita, Osaka 564-8680, Japan.
Biosci Biotechnol Biochem. 2007 Sep;71(9):2198-205. doi: 10.1271/bbb.70158. Epub 2007 Sep 7.
We estimated the nutritional availability of selenium (Se) in Se-enriched Kaiware radish sprouts (SeRS) by the tissue Se deposition and glutathione peroxidase (GPX) activity of rats administered the sprouts, and examined the effect of SeRS on the formation of aberrant crypt foci (ACF) in the colon of mice administered 1,2-dimethylhydrazine (DMH) to evaluate anti-tumor activity. Male weanling Wistar rats were divided into seven groups and fed a Se-deficient basal diet or the basal diet supplemented with 0.05, 0.10, or 0.15 microg/g of Se as sodium selenite or SeRS for 28 d. Supplementation with Se dose-dependently increased serum and liver Se concentrations and GPX activities, and the selenite-supplemented groups showed a higher increase than the SeRS-supplemented groups. The nutritional availability of Se in SeRS was estimated to be 33 or 64% by slope ratio analysis. Male 4-week-old A/J mice were divided into seven groups and fed a low Se basal diet or the basal diet supplemented with selenite, SeRS, or selenite + non-Se-enriched radish sprouts (NonSeRS) at a level of 0.1 or 2.0 microg Se/g for 9 weeks. After 1 week of feeding, all mice were given six subcutaneous injections of DMH (20 mg/kg) at 1-week intervals. The average number of ACF formed in the colon of mice fed the basal diet was 4.3. At a supplementation level of 0.1 mug Se/g, only SeRS significantly inhibited ACF formation. At a supplementation level of 2.0 microg Se/g, both selenite and SeRS significantly inhibited ACF formation. The addition of NonSeRS to the selenite-supplemented diets tended to inhibit ACF formation, but this was not statistically significant. These results indicate that SeRS shows lower nutritional availability but higher anti-tumor activity than selenite.
我们通过给大鼠喂食富硒的水培萝卜芽苗菜（SeRS）后其组织中硒的沉积和谷胱甘肽过氧化物酶（GPX）活性，估算了SeRS中硒（Se）的营养可利用性，并研究了SeRS对给予1,2 - 二甲基肼（DMH）的小鼠结肠中异常隐窝灶（ACF）形成的影响，以评估其抗肿瘤活性。雄性断乳Wistar大鼠分为七组，分别喂食缺硒基础日粮或添加了0.05、0.10或0.15微克/克硒的基础日粮，硒以亚硒酸钠或SeRS的形式添加，持续28天。补充硒后血清和肝脏中的硒浓度及GPX活性呈剂量依赖性增加，且亚硒酸钠补充组的增加幅度高于SeRS补充组。通过斜率比分析估计SeRS中硒的营养可利用性为33%或64%。4周龄雄性A/J小鼠分为七组，分别喂食低硒基础日粮或添加了亚硒酸钠、SeRS或亚硒酸钠 + 非富硒萝卜芽苗菜（NonSeRS）的基础日粮，硒含量为0.1或2.0微克/克，持续9周。喂食1周后，所有小鼠每隔1周皮下注射6次DMH（20毫克/千克）。喂食基础日粮的小鼠结肠中形成的ACF平均数量为4.3个。在硒补充水平为0.1微克/克时，只有SeRS显著抑制ACF形成。在硒补充水平为2.0微克/克时，亚硒酸钠和SeRS均显著抑制ACF形成。在亚硒酸钠补充日粮中添加NonSeRS有抑制ACF形成的趋势，但无统计学意义。这些结果表明，与亚硒酸钠相比，SeRS的营养可利用性较低，但抗肿瘤活性较高。
