Mahalak S M, Lin W L, Essner E, Shichi H
Kresge Eye Institute, Wayne State University School of Medicine, Detroit, MI.
Curr Eye Res. 1991 Nov;10(11):1059-63. doi: 10.3109/02713689109020344.
The immunoreactivity of types I, III and V collagen, fibronectin and transforming growth factor-beta (TGF-beta) was studied by an immunogold labeling method in retinal vessels of rats with experimental autoimmune uveoretinitis (EAU) induced by retinal S-antigen. The basal lamina of retinal capillaries in normal rats showed low immunoreactivities for the extracellular matrix components and TGF-beta. However, at the peak of inflammation (day 13-16 postimmunization), labeling of all three types of collagen and fibronectin increased considerably in the basal lamina of retinal vessels. TGF-beta immunoreactivity was detected mainly in the vascular endothelium and the pericytes. The results suggest that TGF-beta synthesized by the endothelial cells and pericytes may regulate the synthesis and composition of extracellular matrix components in the vascular basal lamina during the course of EAU development.
采用免疫金标记法，研究了视网膜S抗原诱导的实验性自身免疫性葡萄膜视网膜炎（EAU）大鼠视网膜血管中I型、III型和V型胶原蛋白、纤连蛋白及转化生长因子-β（TGF-β）的免疫反应性。正常大鼠视网膜毛细血管的基底膜对细胞外基质成分和TGF-β的免疫反应性较低。然而，在炎症高峰期（免疫后第13 - 16天），视网膜血管基底膜中所有三种胶原蛋白和纤连蛋白的标记显著增加。TGF-β免疫反应性主要在血管内皮细胞和周细胞中检测到。结果表明，内皮细胞和周细胞合成的TGF-β可能在EAU发展过程中调节血管基底膜中细胞外基质成分的合成和组成。
