Fujikawa L S, Chan C C, McAllister C, Gery I, Hooks J J, Detrick B, Nussenblatt R B
Cell Immunol. 1987 Apr 15;106(1):139-50. doi: 10.1016/0008-8749(87)90157-2.
To analyze the role of the retinal vascular endothelial cells in the development of experimental autoimmune uveitis (EAU), we studied the presence of Ia antigen and FN in retinal vessels of Lewis rats immunized with retinal S antigen. Immunopathologic studies were performed on frozen tissues obtained during various stages of the disease. Our results show that Ia antigen was not present in the normal rat retina, and there was very little FN present in a few retinal vessels. One to two days prior to the histologic and clinical onset of EAU, FN was found to be increased in the retinal vessels. Ia antigen was found to be present in the retinal vessels coincident with the first signs of cellular infiltration. During the stage of maximal cellular infiltration, FN was present diffusely throughout the retina, as well as in the subretinal space, and Ia antigen was found diffusely in the cellular infiltrate. Therefore, FN and Ia antigen reflect the immunomodulation of vascular endothelial cells in EAU, which may be very important in the pathogenesis of retinal S antigen-induced uveitis. Two possible mechanisms for the role of the activation of the retinal vascular endothelium in the development of retinal inflammation in uveitis are discussed.
为了分析视网膜血管内皮细胞在实验性自身免疫性葡萄膜炎（EAU）发病过程中的作用，我们研究了用视网膜S抗原免疫的Lewis大鼠视网膜血管中Ia抗原和纤维连接蛋白（FN）的存在情况。对疾病不同阶段获取的冷冻组织进行了免疫病理学研究。我们的结果显示，正常大鼠视网膜中不存在Ia抗原，少数视网膜血管中存在极少量的FN。在EAU组织学和临床发病前1至2天，发现视网膜血管中的FN增加。在视网膜血管中发现Ia抗原与细胞浸润的最初迹象同时出现。在细胞浸润最严重的阶段，FN弥漫分布于整个视网膜以及视网膜下间隙，并且在细胞浸润处广泛发现Ia抗原。因此，FN和Ia抗原反映了EAU中血管内皮细胞的免疫调节作用，这在视网膜S抗原诱导的葡萄膜炎发病机制中可能非常重要。本文讨论了视网膜血管内皮细胞活化在葡萄膜炎视网膜炎症发生过程中作用的两种可能机制。
