Anderson Daniel J, Hetzer Martin W
Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA.
Nat Cell Biol. 2007 Oct;9(10):1160-6. doi: 10.1038/ncb1636. Epub 2007 Sep 9.
The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPgammaS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation.
在后生动物细胞分裂过程中，围绕染色质形成核膜（NE）是一个主要的膜重塑事件。目前尚不清楚核膜是通过NE片段融合而重新形成，还是从完整的内质网（ER）管状网络中重新出现。在这里，我们表明NE的形成和扩张需要一个管状ER网络，并且在存在膜融合抑制剂GTPγS的情况下能够高效发生。膜与染色质的结合由小管末端结合启动，随后形成、扩张和平坦膜片的封闭，这是由内质网中存在的DNA结合蛋白介导的。因此，染色质在核膜形成过程中对内质网的重塑起着积极作用。
