Liu Hongju, Xin Jianbao, Tao Xiaonan, Shang Dan, Zhou Qiong
Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):381-4. doi: 10.1007/s11596-007-0408-3.
In order to investigate the immunity of unloaded dendritic cells (DCs) derived from murine bone marrow to preexisting lung melanoma metastases of mice, MO5 were intravenously injected to induce lung metastases in syngeneic C57BL/6 mice. Unloaded GM-CSF DCs, PBS and DCs+SIINFEKEL were subcutaneously injected into the mice, which were divided as experimental group, negative control group and positive control group respectively. Monoclonal antibody was used to deplete NK or T cells separately. The immunity-inhibitory effects on the lung melanoma were observed and the corresponding effector cells were examined. It was found that in the experimental and positive groups, the regression was induced in metastatic nodules in the lungs of tumor-bearing mice, but abrogated by treatment with anti-asialo-GM1 but not anti-CD8. It was concluded that the unloaded DCs could suppress the lung melanoma metastases to some extent, which was mediated by NK cells, and could be used as a potent therapeutic agents for lung tumor.
为了研究源自小鼠骨髓的未负载树突状细胞（DCs）对小鼠预先存在的肺黑色素瘤转移灶的免疫作用，将MO5静脉注射到同基因C57BL/6小鼠中以诱导肺转移。将未负载的GM-CSF DCs、PBS和DCs+SIINFEKEL分别皮下注射到小鼠体内，这些小鼠分别分为实验组、阴性对照组和阳性对照组。使用单克隆抗体分别清除NK或T细胞。观察对肺黑色素瘤的免疫抑制作用并检测相应的效应细胞。结果发现，在实验组和阳性组中，荷瘤小鼠肺内转移结节出现消退，但用抗去唾液酸GM1处理可消除这种消退，而抗CD8处理则不能。得出结论，未负载的DCs可在一定程度上抑制肺黑色素瘤转移，这是由NK细胞介导的，并且可作为治疗肺肿瘤的有效治疗剂。
