Yin Jie, Liu Yi, Lv Liqun, Wang Donghua, Gong Cheng, Xiao Wei, Sheng Hui
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):461-3. doi: 10.1007/s11596-007-0429-y.
To investigate the expression of mRNA of leptin long-form receptor (OB-Rb) in luteinized granulosa cells of obese women with polycystic ovary syndrome (PCOS), and to determine the role of leptin in the physiopathology of PCOS, luteinized granulosa cells were collected from the follicle fluid of 10 obese women who met the diagnostic criteria for PCOS and their BMI was equal to or greater than 25 kg/m(2), and at the same time, granulosa cells were collected from 10 normal women undergoing IVF-ET who served as the control group. Some luteinized granulosa cells were taken from normal women for in-vitro culture, into which human leptin of different concentrations was added (0, 10, 100 and 1000 ng/mL). After stimulation with leptin for 48 h, RT-PCR was employed for the detection of the expression of OB-RLmRNA in the luteinized granulosa cells. Our results showed that the level of OB-RLmRNA in luteinized granulosa cells of obese PCOS women was higher than those in the control (P<0.05). In luteinized granulosa cells cultured in vitro and stimulated by human leptin for 48 h, the level of OB-RLmRNA was higher than those without leptin stimulation (P<0.01), and when leptin concentration was at 100 ng/mL, and the level of OB-R(L)mRNA reached a peak. It is concluded that in obese PCOS women, the level of serum leptin is increased, which promotes the expression of OB-R(L) in luteinized granulosa cells and increases the sensitivity of the granulosa cells to leptin. Leptin may contribute to anovulation in obese women with PCOS.
为研究多囊卵巢综合征（PCOS）肥胖女性黄素化颗粒细胞中瘦素长型受体（OB-Rb）mRNA的表达情况，并确定瘦素在PCOS病理生理过程中的作用，收集了10名符合PCOS诊断标准且体重指数（BMI）等于或大于25kg/m²的肥胖女性卵泡液中的黄素化颗粒细胞，同时收集了10名接受体外受精-胚胎移植（IVF-ET）的正常女性的颗粒细胞作为对照组。取部分正常女性的黄素化颗粒细胞进行体外培养，向其中加入不同浓度的人瘦素（0、10、100和1000ng/mL）。用瘦素刺激48小时后，采用逆转录聚合酶链反应（RT-PCR）检测黄素化颗粒细胞中OB-RLmRNA的表达。结果显示，肥胖PCOS女性黄素化颗粒细胞中OB-RLmRNA水平高于对照组（P<0.05）。在体外培养并用瘦素刺激48小时的黄素化颗粒细胞中，OB-RLmRNA水平高于未用瘦素刺激的细胞（P<0.01），当瘦素浓度为100ng/mL时，OB-R(L)mRNA水平达到峰值。结论是，肥胖PCOS女性血清瘦素水平升高，促进了黄素化颗粒细胞中OB-R(L)的表达，增加了颗粒细胞对瘦素的敏感性。瘦素可能导致肥胖PCOS女性排卵障碍。
