Lu Xi-Ping, Wang Bo, Huang He-Feng
Dongyang Municipal People's Hospital, Dongyang 322100, China.
Zhonghua Yi Xue Za Zhi. 2005 Jan 12;85(2):88-91.
OBJECTIVE: To investigate the expression of leptin mRNA in luteinized granulose cells and the leptin level in serum and follicular fluid of non-obese patients with polycystic ovary syndrome (PCOS). METHODS: Granulosa cells were collected from the puncture fluid of follicle of 16 obese infertile patients with PCOS to undergo external fertilization. Fasting venous blood was collected while collecting the ova. RT-PCR was used to detect the expression of leptin mRNA in the granulosa cells. ELISA was used to detect the leptin level in the serum and follicular fluid. Twenty normal women undergoing external fertilization because of male infertility of their husbands were used as controls. RESULTS: The expression of leptin mRNA (A value) was 1.5 +/- 0.4 in the obese infertile patients with PCOS and was 1.8 +/- 0.6 (P > 0.05) in the controls. Both the leptin levels of serum and follicular fluid were not different between the obese infertile patients with PCOS and controls (both P > 0.05). The leptin level in follicular fluid was significantly correlated with both the serum leptin level in the obese infertile patients with PCOS (r(s) = 0.838, P = 0.000) and the control group (r(s) = 0.712, P = 0.000). The expression level of leptin mRNA in the luteinized was not correlated with the leptin level of follicular fluid in both the obese infertile patients with PCOS and the control group (both P > 0.05). The fertility rate was not different between the obese infertile patients with PCOS (7/16) and the controls (9/24) (P = 0.693). CONCLUSION: The expression of leptin mRNA is normal in the follicular fluid of the obese infertile patients with PCOS. The leptin levels in serum and follicular fluid of the obese infertile patients with PCOS are similar to those of normal women. The leptin level in follicular fluid depends mainly on the circulating leptin level.
目的：研究非肥胖型多囊卵巢综合征（PCOS）患者黄素化颗粒细胞中瘦素mRNA的表达以及血清和卵泡液中瘦素水平。 方法：收集16例因肥胖不孕而行体外受精的PCOS患者卵泡穿刺液中的颗粒细胞。在采集卵子时同时采集空腹静脉血。采用逆转录-聚合酶链反应（RT-PCR）检测颗粒细胞中瘦素mRNA的表达。采用酶联免疫吸附测定（ELISA）检测血清和卵泡液中瘦素水平。选取20例因丈夫男性不育而行体外受精的正常女性作为对照。 结果：肥胖不孕PCOS患者瘦素mRNA表达（A值）为1.5±0.4，对照组为1.8±0.6（P>0.05）。肥胖不孕PCOS患者与对照组血清和卵泡液中瘦素水平均无差异（均P>0.05）。肥胖不孕PCOS患者卵泡液中瘦素水平与血清瘦素水平显著相关（斯皮尔曼等级相关系数r(s)=0.838，P=0.000），对照组中两者也显著相关（r(s)=0.712，P=0.000）。肥胖不孕PCOS患者和对照组中，黄素化颗粒细胞中瘦素mRNA表达水平与卵泡液中瘦素水平均无相关性（均P>0.05）。肥胖不孕PCOS患者（7/16）与对照组（9/24）的妊娠率无差异（P=0.693）。 结论：肥胖不孕PCOS患者卵泡液中瘦素mRNA表达正常。肥胖不孕PCOS患者血清和卵泡液中瘦素水平与正常女性相似。卵泡液中瘦素水平主要取决于循环中的瘦素水平。
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2006-10
J Huazhong Univ Sci Technolog Med Sci. 2007-8
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