Medlock Kathryn, Harmer Hazel, Worsley Graham, Horgan Adrian, Pritchard John
Smart Holograms Ltd., 291 Cambridge Science Park, Milton Road, Cambridge CB4 0WF, UK.
Anal Bioanal Chem. 2007 Nov;389(5):1533-9. doi: 10.1007/s00216-007-1548-9. Epub 2007 Sep 9.
Conventional electrochemical methods of determining the pH of body fluids have drawbacks. Newer optical methods offer the promise of miniaturisation and continuous in vivo measurements without drift. This report examines the ability of a holographic sensor based on a thin-film, biocompatible hydrogel (approximately 10 microm) of poly(2-hydroxyethyl methacrylate) and ionisable 2-(dimethylaminoethyl) methacrylate to accurately measure the pH of blood plasma ex vivo. It is found that the sensors behave in a fully reversible manner. After an initial calibration with buffers, they can measure pH over extended periods (more than 40 h).
传统的测定体液pH值的电化学方法存在缺陷。更新的光学方法有望实现小型化，并能在体内进行连续测量且无漂移现象。本报告研究了一种基于聚（甲基丙烯酸2-羟乙酯）和可电离的2-（二甲氨基乙基）甲基丙烯酸酯的薄膜生物相容性水凝胶（约10微米）的全息传感器在体外准确测量血浆pH值的能力。结果发现，这些传感器具有完全可逆的行为。在用缓冲液进行初始校准后，它们能够长时间（超过40小时）测量pH值。
