Sartain Felicity K, Yang Xiaoping, Lowe Christopher R
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK.
Anal Chem. 2006 Aug 15;78(16):5664-70. doi: 10.1021/ac060416g.
Measurement of blood l-lactate is used to assess and monitor exercise performance in sports medicine. This report describes the initial development of a holographic sensor, which employs a synthetic receptor, to enable the selective and continuous real-time measurement of l-lactate for eventual in vivo application. Three boronic acid-based receptors have been synthesized, integrated into thin acrylamide hydrogel films, and then subsequently transformed into holographic sensors. Changes in the replay wavelength of the sensors were used to characterize the swelling behavior of the matrix as a function of l-lactate concentration. It was found that the incorporation of 3-acrylamidophenyl boronic acid into an acrylamide hydrogel produced the largest response toward l-lactate. The effects of hydrogel composition, fluctuating l-lactate concentrations, and the response of potential interfering agents to the sensor have been investigated.
血液中L-乳酸的测量用于运动医学中评估和监测运动表现。本报告描述了一种全息传感器的初步开发，该传感器采用合成受体，能够选择性地连续实时测量L-乳酸，最终用于体内应用。已合成了三种基于硼酸的受体，将其整合到丙烯酰胺水凝胶薄膜中，然后转化为全息传感器。传感器重放波长的变化用于表征基质作为L-乳酸浓度函数的溶胀行为。结果发现，将3-丙烯酰胺基苯硼酸掺入丙烯酰胺水凝胶中对L-乳酸产生的响应最大。研究了水凝胶组成、L-乳酸浓度波动以及潜在干扰剂对传感器的响应的影响。
