Zhuo Shuangmu, Chen Jianxin, Jiang Xingshan, Luo Tianshu, Chen Rong, Xie Shusen, Zou Qilian
Key Laboratory of Optoelectronic Science and Technology for Medicine, (Fujian Normal University), Ministry of Education, Fuzhou, China.
Scanning. 2007 Sep-Oct;29(5):219-24. doi: 10.1002/sca.20066.
We demonstrate the technique of subsequent multitrack nonlinear imaging based on backscattered second-harmonic generation (B-SHG) and two-photon autofluorescence (TPA) to obtain large-area, high-contrast, submicron-resolution image ex vivo of esophageal stroma. Our findings show that this technique is effective in improving the B-SHG/TPA image contrast. It was found that the method can quantitatively obtain microscopic structural and biochemical information on stroma. Our work suggests that the technique has the potential to provide accurate and comprehensive information in determining the physiological and pathological states of the esophagus.
我们展示了基于背向散射二次谐波产生（B-SHG）和双光子自发荧光（TPA）的后续多通道非线性成像技术，以在体外获得大面积、高对比度、亚微米分辨率的食管基质图像。我们的研究结果表明，该技术在提高B-SHG/TPA图像对比度方面是有效的。发现该方法可以定量获取关于基质的微观结构和生化信息。我们的工作表明，该技术在确定食管的生理和病理状态方面有潜力提供准确而全面的信息。
