Okada Tetsuo
Department of Chemistry, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8551, Japan.
Electrophoresis. 2007 Oct;28(19):3414-9. doi: 10.1002/elps.200700102.
Liquid-core waveguide (LCW) brings about several advantages in CE. This review discusses some aspects of fundamental and practical importance involved in this method. Sensitivity in absorption and fluorescence detection is in general improved by more than one order of magnitude over usual crossbeam detection arrangements; the improvements come from the long light path in absorption detection and low light scattering in fluorescence detection. Versatile instrumental arrangements are another advantage of LCW in CE, leading to several detection schemes, some of which provide information that is not gained by usual capillary-end crossbeam detection, e.g. whole-capillary imaging, simultaneous monitoring of multicapillary separation, and kinetic evaluation. The high potential and perspectives of LCW in CE are discussed based on the state-of-the-art developments.
液芯波导（LCW）在毛细管电泳（CE）中具有诸多优势。本综述探讨了该方法在基础和实际应用方面的一些重要内容。与常规的交叉光束检测装置相比，吸收和荧光检测的灵敏度通常提高了一个以上数量级；这些改进源于吸收检测中的长光路以及荧光检测中的低光散射。通用的仪器配置是LCW在CE中的另一个优势，它带来了多种检测方案，其中一些方案能提供常规毛细管末端交叉光束检测无法获得的信息，例如全毛细管成像、多毛细管分离的同步监测以及动力学评估。基于当前的前沿进展，讨论了LCW在CE中的巨大潜力和前景。
