Caglar Perihan, Landers James P
Department of Chemistry, Hacettepe University, Ankara, Turkey.
J Capill Electrophor Microchip Technol. 2003 May-Aug;8(3-4):69-76.
This article evaluates the potential of using fiber optics as a detection tool during electrophoretic separations or identification and quantification of analytes in miniaturized systems, including microchips. This paper covers reports in the literature from 1989 to June 2002. The articles have been chosen based on various aspects of the instrumentation including the microchip substrates, channel size and geometry, pathlength, fiber and detector types, light source, separation conditions, detection limits, and applications-all with the goal of comparing and contrasting optical detections. This review has been divided into three groups: fluorescence measurement-based studies, absorbance measurement-based studies, and innovative studies in chronological order.
本文评估了在电泳分离过程中使用光纤作为检测工具的潜力，以及在包括微芯片在内的小型化系统中对分析物进行鉴定和定量的潜力。本文涵盖了1989年至2002年6月期间文献中的报道。这些文章是根据仪器的各个方面挑选出来的，包括微芯片基板、通道尺寸和几何形状、光程、光纤和探测器类型、光源、分离条件、检测限以及应用——所有这些都是为了比较和对比光学检测。本综述按时间顺序分为三组：基于荧光测量的研究、基于吸光度测量的研究和创新性研究。
