Das O P, Ward K, Ray S, Messing J
Waksman Institute, Rutgers State University of New Jersey, Piscataway 08855-0759.
Genomics. 1991 Dec;11(4):849-56. doi: 10.1016/0888-7543(91)90007-2.
In many inbred lines of maize, two 27-kDa storage protein (zein) genes are found within tandem duplications of 12 kb. Both genes of the duplicated allele from the maize inbred line A188 were sequenced and compared to a similar duplicated allele in another inbred line, W22, and to a single-copy allele in the inbred line W64A. The comparisons reveal interesting patterns in the distribution of sequence changes between these alleles. Differences between the two duplicated alleles that are conserved between the two genes of each allele are found exclusively in the 5' region. In contrast, differences between the individual genes of each allele in the 3' region are conserved between the two alleles. The first case is indicative of an intraallelic copy correction mechanism, whereas the second may result from interallelic copy correction. These may be mediated by gene conversion processes, as previously described for other multigene families.
在许多玉米自交系中，在12 kb的串联重复序列中发现了两个27 kDa的贮藏蛋白（玉米醇溶蛋白）基因。对玉米自交系A188的重复等位基因的两个基因进行了测序，并与另一个自交系W22中的类似重复等位基因以及自交系W64A中的单拷贝等位基因进行了比较。这些比较揭示了这些等位基因之间序列变化分布的有趣模式。每个等位基因的两个基因之间保守的两个重复等位基因之间的差异仅在5'区域发现。相反，每个等位基因在3'区域的单个基因之间的差异在两个等位基因之间是保守的。第一种情况表明存在等位基因内拷贝校正机制，而第二种情况可能是等位基因间拷贝校正的结果。这些可能由基因转换过程介导，如先前针对其他多基因家族所描述的那样。
