Science. 1963 Dec 27;142(3600):1655-6. doi: 10.1126/science.142.3600.1655.
Barley embryos, measuring 90 Fz in length, grew and became differentiated on a phosphate-enriched White's medium which was fortified with glutamine and alanine as major sources of organic nitrogen, and lesser amounts of five other amino acids. The pH of the medium was a critical factor in differentiation. On this medium, excised barley embryos developed more rapidly than embryos remaining in vivo, or embryos grown on media containing coconut milk.
胚长 90μm 的大麦幼胚在含有谷氨酰胺和丙氨酸的 White 培养基上生长并分化，该培养基为有机氮的主要来源，还添加了少量其他五种氨基酸。培养基的 pH 值是分化的关键因素。在这种培养基上，离体大麦幼胚的发育速度比体内幼胚或在含椰子乳的培养基上生长的幼胚快。
