Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, FRG.
Plant Cell Rep. 1985 May;4(3):168-71. doi: 10.1007/BF00571309.
Because most of the intergenericGramineae embryos abort before they can be isolated and cultured, our object was to grow ovules at an early stage of embryogenesis. Ovules were at size 1 to 7 mm. The youngest stages represented ovules containing several-celled proembryos; the oldest stage consisted of embryos at the level of differentiation of the organs. 1357 ovules were jointly inoculated in 12 different media of which only 3 appeared to be most suitable for the growth and differentiation of globular proembryos. From several-celled proembryos (ovules at the size of 1.5 - 2.5 mm) only compact calluses developed. The capability of proembryos to differentiate and to form fully developed embryos and consequently plants started when the ovules were inoculated at the size of 2.5 - 3.0 mm. Since the medium plays an important role in the process of differentiation of proembryos, the application of similar culture conditions is suggested for the in vitro culture of haploid or hybrid proembryos obtained among wide crosses inGramineae.
由于大多数属间禾本科胚胎在能够被分离和培养之前就已经流产，我们的目标是在胚胎发生的早期阶段生长胚珠。胚珠的大小为 1 至 7 毫米。最小的阶段代表含有几个细胞原胚的胚珠；最老的阶段包括处于器官分化水平的胚胎。1357 个胚珠共同接种在 12 种不同的培养基中，只有 3 种培养基似乎最适合球形原胚的生长和分化。从多细胞原胚（大小为 1.5-2.5 毫米的胚珠）中仅形成了致密的愈伤组织。当胚珠接种大小为 2.5-3.0 毫米时，原胚开始分化并形成完全发育的胚胎和随后的植物。由于培养基在原胚分化过程中起着重要作用，因此建议在禾本科中广泛杂交获得的单倍体或杂种原胚的体外培养中应用类似的培养条件。
