Yamakawa Y, Omori-Satoh T
Department of Applied Immunology, National Institute of Health, Tokyo.
J Biochem. 1991 Nov;110(5):767-9. doi: 10.1093/oxfordjournals.jbchem.a123656.
A new method is presented for purifying antihemorrhagic factor in the serum of Japanese Habu snake (Trimeresurus flavoviridis). The method consists of specific binding of the factor to a hemorrhagic principle-conjugated Sepharose, gel filtration and reverse-phase HPLC. The improved method is simple and rapid, providing the factor with a great increase in specific activity and in a high yield.