Tomihara Y, Yonaha K, Nozaki M, Yamakawa M, Kamura T, Toyama S
Toxicon. 1987;25(6):685-9. doi: 10.1016/0041-0101(87)90117-6.
Three antihemorrhagic factors (AHF-1, AHF-2 and AHF-3) were purified from the serum of H. edwardsii, a mongoose, by a combination of gel filtration on a Sephadex G-200 column and high performance liquid chromatography with a TSK gel DEAE-5PW column. Each of the purified antihemorrhagic factors showed a single band on polyacrylamide gel disc electrophoresis. The three antihemorrhagic factors inhibited the hemorrhagic activity of HR 1 and HR 2, the hemorrhagic principles from the snake venom of Trimeresurus flavoviridis Okinawa. AHF-1, AHF-2 and AHF-3 were stable at temperatures from 0 degrees to 60 degrees C and at pH values between 2.0 and 11.0. The same molecular weight (65,000) was obtained for the three antihemorrhagic factors. No precipitin lines were found for the purified antihemorrhagic factors with the venom of T. flavoviridis Okinawa and its hemorrhagic principles, HR 1 and HR 2.
通过在Sephadex G - 200柱上进行凝胶过滤和使用TSK凝胶DEAE - 5PW柱进行高效液相色谱相结合的方法，从食蟹猴血清中纯化出三种抗出血因子（AHF - 1、AHF - 2和AHF - 3）。每种纯化后的抗出血因子在聚丙烯酰胺凝胶圆盘电泳上均显示出一条带。这三种抗出血因子抑制了绿竹蛇（Trimeresurus flavoviridis Okinawa）蛇毒中的出血成分HR 1和HR 2的出血活性。AHF - 1、AHF - 2和AHF - 3在0摄氏度至60摄氏度的温度范围内以及pH值在2.0至11.0之间时均稳定。这三种抗出血因子的分子量相同（65,000）。纯化后的抗出血因子与绿竹蛇（Trimeresurus flavoviridis Okinawa）的毒液及其出血成分HR 1和HR 2未发现沉淀线。
