Watanabe H, Sugahara K, Inoue K, Fujita Y, Kodama H
Department of Urology, Kochi Medical School, Japan.
J Chromatogr. 1991 Aug 23;568(2):445-50. doi: 10.1016/0378-4347(91)80182-c.
Analyses of amino acids in the urine of a normal human and of patients with heterozygous and homozygous cystinuria have been carried out, using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface system. A kidney cystine stone was also analysed by this system. Very intense quasi-molecular ions ([M + H]+) of standard cystine, arginine, lysine and ornithine were observed on mass chromatograms as base peaks. Mass chromatograms of the urine samples from a normal human and from patients with heterozygous and homozygous cystinuria were easily distinguishable. The retention times in the mass chromatogram and mass spectrum of kidney stone cystine was almost the same as that of authentic cystine.
利用带有大气压电离接口系统的液相色谱 - 质谱联用技术，对正常人和杂合子及纯合子胱氨酸尿症患者的尿液中的氨基酸进行了分析。还用该系统对一块肾胱氨酸结石进行了分析。在质量色谱图上，标准胱氨酸、精氨酸、赖氨酸和鸟氨酸的非常强的准分子离子（[M + H]+）作为基峰被观测到。来自正常人以及杂合子和纯合子胱氨酸尿症患者的尿液样本的质量色谱图很容易区分。肾结石胱氨酸在质量色谱图和质谱中的保留时间与纯胱氨酸几乎相同。
