Science. 1965 Nov 12;150(3698):889-92. doi: 10.1126/science.150.3698.889.
Single cells isolated from pith callus of fresh stem of hybrid tobacco (Nicotiana glutinosa male symbol x N. tabacum female symbol) gave rise to small colonies of cells in microcultures which upon transfer to agar medium produced clones of callus tissue. These single-cell clones differentiated roots, and shoots with green leaves, on a completely defined nutrient medium. The rooted shoots developed into normal plants after transfer to soil in pots in the greenhouse. Buds and flowers were produced by these plants.
从杂交烟草（雄性不育菸草品种 x 雌性可育菸草品种）新鲜茎的髓部愈伤组织中分离出来的单细胞在微培养中会形成小细胞集落，这些小细胞集落转移到琼脂培养基上后会产生愈伤组织的克隆。这些单细胞克隆在完全定义的营养培养基上分化出根和带绿叶的芽。生根的芽在温室盆栽土壤中转移后发育成正常植物。这些植物产生了芽和花。
