Hioki A, Ohtomo H
Department of Parasitology, Gifu University School of Medicine.
Kansenshogaku Zasshi. 1991 Dec;65(12):1508-13. doi: 10.11150/kansenshogakuzasshi1970.65.1508.
The effects of antimalarial treatment on the blood oxygen-transporting properties and on the tissue hypoxia were investigated in severe murine malaria, using mice infected with Plasmodium berghei (NK65). Five week old male ddY mice were inoculated intraperitoneally with 1 X 10(7) of P. berghei-infected red blood cells and treated with Fansidar (20 mg/kg body weight sulfadoxine and 1 mg/kg body weight pyrimethamine orally) on day 5 after inoculation. Parasitemia in these mice decreased rapidly on day 1 after treatment. Blood hemoglobin concentration, however, decreased on days 1 and 2 of treatment, then began to increase. The actual oxygen equilibrium curve (OEC) in vivo (actual pH; actual Pco2; 36.5 degrees C) was calculated from the measured OEC and the results of blood gas analysis. Looking from arterial and venous Po2 of each group, blood oxygen-transporting properties decreased markedly on day 2 of treatment. This decrease resulted mainly from the decrease of hemoglobin concentration and also partly from the raised hemoglobin affinity for oxygen. Adenosine triphosphate concentration in liver tissues, however, began to increase on day 1 of treatment. Adenylate energy charge of liver tissues also recovered on day 1. Blood glucose concentration began to increase and blood lactate concentration began to decrease simultaneously on day 1 of treatment. Glucose concentration in liver tissues, in contrast, decreased on days 1 and 2 of inoculation. Lactate concentration in liver tissue decreased earlier on day 1. These data indicate that tissue hypoxia was removed on day 1 following antimalarial treatment although blood oxygen-transporting properties decreased on days 1 and 2 after treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
在重度鼠疟模型中，使用感染伯氏疟原虫（NK65）的小鼠，研究了抗疟治疗对血液氧运输特性和组织缺氧的影响。5周龄雄性ddY小鼠腹腔注射1×10⁷个感染伯氏疟原虫的红细胞，并在接种后第5天用Fansidar（磺胺多辛20mg/kg体重和乙胺嘧啶1mg/kg体重，口服）进行治疗。治疗后第1天，这些小鼠的疟原虫血症迅速下降。然而，治疗第1天和第2天，血液血红蛋白浓度下降，随后开始上升。根据实测的氧平衡曲线（OEC）和血气分析结果，计算了体内实际氧平衡曲线（实际pH；实际Pco₂；36.5℃）。从每组的动脉和静脉血氧分压来看，治疗第2天血液氧运输特性明显下降。这种下降主要是由于血红蛋白浓度降低，也部分是由于血红蛋白对氧的亲和力升高。然而，肝组织中的三磷酸腺苷浓度在治疗第1天开始升高。肝组织的腺苷酸能荷在第1天也恢复了。治疗第1天，血糖浓度开始升高，同时血乳酸浓度开始下降。相比之下，接种后第1天和第2天肝组织中的葡萄糖浓度下降。肝组织中的乳酸浓度在第1天更早下降。这些数据表明，抗疟治疗后第1天组织缺氧被消除，尽管治疗后第1天和第2天血液氧运输特性下降。（摘要截选至250字）
