Koivula T, Hemilä H
Institute of Biotechnology, University of Helsinki, Finland.
J Gen Microbiol. 1991 Nov;137(11):2595-600. doi: 10.1099/00221287-137-11-2595.
We have previously isolated a putative promoter from the Lactococcus lactis subsp. lactis chromosome. We now report the sequence of the promoter fragment and its extension in the 5'-direction. The region contains several open-reading frames which correspond to ribosomal protein L15, SecY, adenylate kinase, initiation factor 1 and ribosomal proteins B and S13. The order of the genes, rplO (L15), secY, adk, infA, rpmJ (B) and rpsM (S13), is similar to that in the spc and alpha operon region of Bacillus subtilis, with the exception of the map gene, coding for methionine amino peptidase, which is located between adk and infA in B. subtilis. The putative promoter is located between adk and infA.
我们之前已从乳酸乳球菌乳酸亚种染色体中分离出一个假定的启动子。现在我们报告该启动子片段的序列及其在5'方向上的延伸。该区域包含几个开放阅读框，分别对应核糖体蛋白L15、SecY、腺苷酸激酶、起始因子1以及核糖体蛋白B和S13。这些基因的顺序，即rplO（L15）、secY、adk、infA、rpmJ（B）和rpsM（S13），与枯草芽孢杆菌的spc和α操纵子区域中的顺序相似，但枯草芽孢杆菌中编码甲硫氨酸氨基肽酶的map基因位于adk和infA之间，而此处没有该基因。假定的启动子位于adk和infA之间。
