Oshima K, Gorbman A, Shimada H
Science. 1969 Jul 4;165(3888):86-8. doi: 10.1126/science.165.3888.86.
Homing salmon were injected intracranially with puromycin, actinomycin D, or cycloheximide. From 4 to 7 hours after such treatment these agents markedly inhibited olfactory bulbar discrimination between home water and other natural waters, including spawning sites for other groups of salmon. At longer intervals after treatment there was a partial restoration of olfactory memory-based discrimination. The dosages of the inhibitors used could be shown to have depressed incorporation of H(3)-leucine into protein by 78 percent or of H(3)-uridine into RNA by 41 percent in the salmon brains 4 hours after intracranial injection. These findings suggest that acute blockage of RNA synthesis or protein synthesis can interfere with long-term olfactory memory in anadromous salmon, at least as this function can be analyzed by electrophysiological methods. This implies that long-term olfactory memory depends upon continued metabolism of RNA and continued protein synthesis.
对洄游的鲑鱼进行颅内注射嘌呤霉素、放线菌素D或环己酰亚胺。在这种处理后的4至7小时内，这些药剂显著抑制了嗅球对原居地水域和其他天然水域（包括其他鲑鱼群体的产卵地）之间的辨别能力。处理后较长时间间隔时，基于嗅觉记忆的辨别能力会部分恢复。所使用抑制剂的剂量显示，在颅内注射4小时后，鲑鱼大脑中H(3)-亮氨酸掺入蛋白质的量减少了78%，H(3)-尿苷掺入RNA的量减少了41%。这些发现表明，RNA合成或蛋白质合成的急性阻断会干扰溯河产卵鲑鱼的长期嗅觉记忆，至少就这种功能可通过电生理方法进行分析而言是如此。这意味着长期嗅觉记忆依赖于RNA的持续代谢和蛋白质的持续合成。
