枯草芽孢杆菌 DNA 两条互补链的转录活性。
Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA.
出版信息
Science. 1967 Aug 18;157(3790):817-9. doi: 10.1126/science.157.3790.817.
PMID:17842787
Abstract
Under conditions favoring single-strand transformation, the two complementary strands of Bacillus subtilis DNA, separated by differential complexing with polyriboguanylic acid, have identical transforming activity. Moreover separated single strands, upon renaturation with unmarked (recipient) DNA, form heteroduplex molecules with similar double-strand transformin activity. These findings bear upon the mechanism of DNA integration.
摘要
在有利于单链转化的条件下，枯草芽孢杆菌 DNA 的两条互补链通过与聚鸟苷酸的差异复合而分离，具有相同的转化活性。此外，分离的单链在与未标记的（受体）DNA 复性时，形成具有类似双链转化活性的异源双链分子。这些发现与 DNA 整合的机制有关。
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